Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria

Comparison of family 9 cellulases from mesophilic and thermophilic bacteria.

Cellulases containing a family 9 catalytic domain and a family 3c cellulose binding module (CBM3c) are important components of bacterial cellulolytic systems. We measured the temperature dependence of the activities of three homologs: Clostridium cellulolyticum Cel9G, Thermobifida fusca Cel9A, and C. thermocellum Cel9I. To directly compare their catalytic activities, we constructed six new vers...

Characterization and stability of ribosomes from mesophilic and thermophilic bacteria.

Ribosomes were isolated from three mesophilic and three thermophilic strains of Bacillus. The ribosomes consisted of about 55% protein and 45% ribonucleic acid. Average ratios for the absorbance at 260/235 and 260/280 mmu were 1.77 and 1.92 for the mesophiles and 1.63 and 1.84 for the thermophiles. Ultracentrifugation revealed mainly components with sedimentation coefficients of about 30, 50, 7...

Stability of the ‘L12 stalk’ in ribosomes from mesophilic and (hyper)thermophilic Archaea and Bacteria

The ribosomal stalk complex, consisting of one molecule of L10 and four or six molecules of L12, is attached to 23S rRNA via protein L10. This complex forms the so-called 'L12 stalk' on the 50S ribosomal subunit. Ribosomal protein L11 binds to the same region of 23S rRNA and is located at the base of the 'L12 stalk'. The 'L12 stalk' plays a key role in the interaction of the ribosome with trans...

Effect of nutritional and environmental factors on cellulases activity by thermophilic bacteria isolated from hot spring

This study presents effects of some nutritional and environmental factors on cellulases production by isolates, Bacillus licheniformis WBS1 and Bacillus sp. WBS3, which have been isolated from an Indian hot spring. Activity of both CMCase and FPase were higher in case of wheat straw. Maximum enzymatic activity was found at following optimum parameters: pH (8.0 when grown on rice, 9.0 when grown...

Cellulosomes from mesophilic bacteria.